Table 2. Identification of mucin binding proteins using a homology search of N-terminal amino acid sequences.
Strain | No. identified bands1 |
Determined N-terminal sequences | Homology (%) | Homologous protein | Species | Deduced molecular weight2 |
Accession No. |
---|---|---|---|---|---|---|---|
MYU 208 | 1 | AKDIKFSEDARSKMQAGVDQ | 100 | GroEL | W. viridescens and others | 57,300 | WP_057745829.1 etc. |
2-1 | SAITDIYAREVLDSRGNPTV | 100 | Enolase | W. viridescens and others | 47,900−49,100 | WP_057746967.1 etc. | |
2-2 | AKETYERTKPHVNIGTIGHV | 90−100 | Elongation factor Tu (EF-Tu) | W. viridescens and others | 43,400 | WP_057747191.1 etc. | |
MYU 221 | 3-1 | VQIDNEDLKQVRSEFLDTAK | 100 | Peptidase C1 (PepC) | L. johnsonii and others | 51,500−51,600 | WP_004896885.1 etc. |
3-2 | MLKSVIENVHALEIFDSRGN | 100 | Enolase | L. johnsonii and others | 46,700−46,800 | WP_004897160.1 etc. | |
3-3 | SENEKFEQNAYAPMKGIKVI | 75−90 | Formyl-CoA transferase (FRC) | L. johnsonii and others | 50,600 | WP_004898834.1 etc. | |
4-1 | AKLVLIRHGQSEANLTNQFV | 89 | Phosphoglyceromutase (PGM) | L. johnsonii and others | 26,100 | WP_053106914.1 etc. | |
4-2 | SRTPIIAGNWKLHMNPEQTV | 95−100 | Triosephosphate isomerase (TPI) | L. johnsonii and others | 27,600 | WP_012846434.1 etc. | |
4-3 | MIYTVTVNPALDYVMQLEKV | 100 | Phosphofructokinase (PFK) | L. johnsonii and others | 32,500−32,800 | WP_012846697.1 etc. | |
MYU 227 | 5 | SKIIGIDLGTTNSAVAVLQG | 95 | DnaK | L. agilis and others | 66,000−67,400 | WP_056976892.1 etc. |
6-1 | SAITEIYAREVLDSRGNPTV | 100 | Enolase | L. agilis and others | 47,800−48,100 | WP_050611677.1 etc. | |
6-2 | AKLIVSDLDVKDKKVLIRVD | 100 | Phosphoglycerate kinase (PGK) | L. agilis and others | 42,200−42,900 | WP_050611679.1 etc. |
1See Fig. 3.
2Deduced from amino acid sequences (simple protein) by the pI/Mw tool.