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. 2016 Aug 2;35(4):185–196. doi: 10.12938/bmfh.16-012

Table 2. Identification of mucin binding proteins using a homology search of N-terminal amino acid sequences.

Strain No. identified
bands1
Determined N-terminal sequences Homology (%) Homologous protein Species Deduced
molecular weight2
Accession No.
MYU 208 1 AKDIKFSEDARSKMQAGVDQ 100 GroEL W. viridescens and others 57,300 WP_057745829.1 etc.
2-1 SAITDIYAREVLDSRGNPTV 100 Enolase W. viridescens and others 47,900−49,100 WP_057746967.1 etc.
2-2 AKETYERTKPHVNIGTIGHV 90−100 Elongation factor Tu (EF-Tu) W. viridescens and others 43,400 WP_057747191.1 etc.

MYU 221 3-1 VQIDNEDLKQVRSEFLDTAK 100 Peptidase C1 (PepC) L. johnsonii and others 51,500−51,600 WP_004896885.1 etc.
3-2 MLKSVIENVHALEIFDSRGN 100 Enolase L. johnsonii and others 46,700−46,800 WP_004897160.1 etc.
3-3 SENEKFEQNAYAPMKGIKVI 75−90 Formyl-CoA transferase (FRC) L. johnsonii and others 50,600 WP_004898834.1 etc.
4-1 AKLVLIRHGQSEANLTNQFV 89 Phosphoglyceromutase (PGM) L. johnsonii and others 26,100 WP_053106914.1 etc.
4-2 SRTPIIAGNWKLHMNPEQTV 95−100 Triosephosphate isomerase (TPI) L. johnsonii and others 27,600 WP_012846434.1 etc.
4-3 MIYTVTVNPALDYVMQLEKV 100 Phosphofructokinase (PFK) L. johnsonii and others 32,500−32,800 WP_012846697.1 etc.

MYU 227 5 SKIIGIDLGTTNSAVAVLQG 95 DnaK L. agilis and others 66,000−67,400 WP_056976892.1 etc.
6-1 SAITEIYAREVLDSRGNPTV 100 Enolase L. agilis and others 47,800−48,100 WP_050611677.1 etc.
6-2 AKLIVSDLDVKDKKVLIRVD 100 Phosphoglycerate kinase (PGK) L. agilis and others 42,200−42,900 WP_050611679.1 etc.

1See Fig. 3.

2Deduced from amino acid sequences (simple protein) by the pI/Mw tool.