FIG. 5.

Changes in expression levels of pyruvate kinase, HSP60, and 14-3-3ζ in response to metabolic stress are mitigated by protein deglutathionylation. THP-1 monocytes were infected with a doxycycline (Dox)-inducible adenoviral vector carrying the sequence for a Grx1-EGFP fusion protein (pAd-Grx1), and Grx1 expression was induced by treating cells for 24 h with 1 μg/ml doxycycline (Dox). THP-1 monocytes were then treated for 24 h with vehicle or were metabolically stressed with human LDL plus 20 mM D-glucose (L-H). Protein levels were measured by Western blot analysis and results are shown as mean ± SE (n = 4). HSP, heat shock protein.