Figure 6.

15d-PGJ₂ altered the profile of CD4⁺CD25⁻ cells under polarizing conditions. Isolated CD4⁺CD25⁻ cells from naïve mice were cultured under Treg ((a)/(b)) or Th17 ((c)/(d)) polarizing conditions with or without 15d-PGJ₂ (5 μM). Natural Treg (nTreg, CD4⁺CD25⁺) or Th0 (CD4⁺CD25⁻) cells were used as positive (for Treg) and negative differentiation controls. The bars represent the percentage of TCD4⁺ cells expressing FOXP3 or IL-17. In (d), IL-17 levels were measured into supernatant culture from Th17 polarizing condition by ELISA assay. The results are expressed as the mean ± SEM obtained from triplicate samples from one of three independent experiments (N = 3 per group). ^∗ P < 0.05 relative to the vehicle group. ^∗ P < 0.05 compared with Th0; ^# P < 0.05 compared with iTreg (b). ^∗ P < 0.05 compared with Th0; ^# P < 0.05 compared with Th17 (c-d).