Figure 4.

Effect of epothilone B on axon growth and cell viability in adult DRG neurons. (a–c) Adult DRG neurons were plated at a low density and cultured in the absence or presence of various concentrations of epothilone B, from 1 pM to 1 μM, as indicated. Neurons were fixed at 24 hr after plating and immunostained with anti-Tuj1 antibodies. Representative images ((a), inverted images from Tuj1 immunostaining) and quantification of axon length (b) are shown. Cell viability was assayed by PI staining (c). Scale bar, 100 μm. ^∗ p < 0.05, ^∗∗ p < 0.01, and ^∗∗∗ p < 0.001; ns, statistically not significant.