Figure 6.

Neurons of different type and age contain MTs that differ in stability. (a–d) Embryonic (E15.5) cortical neurons, embryonic (13.5) DRG neurons, or adult DRG neurons were cultured in the absence or presence of nocodazole (Ncd) (50 nM, 1.5 hr) and were immunostained with anti-Tuj1 antibodies. Representative images (a, d) and quantification of fluorescence intensity of βIII-tubulin immunostaining (b), axon length (c), and percentage of neurons with disrupted MTs (e) are shown. Scale bar, 50 μm. ^∗ p < 0.05, ^∗∗ p < 0.01, and ^∗∗∗ p < 0.001; ns, statistically not significant.