Figure 2. Loss of Cav-1 results in increased IOP and reduced aqueous humor drainage.

(a) Localization of Cav-1 (green) and Cav-2 (red) in murine outflow pathway is similar to that observed in human tissue. CD31 (blue) labels the SC endothelium. (b) Intraocular pressure was significantly increased in Cav1^−/− mice compared to controls as measured by rebound tonometry at indicated ages (*p ≤ 0.05, ** p ≤ 0.01, one-way ANOVA and Newman-Keuls post hoc analysis, n = 13–22 mice for each group). (c) Conventional, pressure-dependent outflow is significantly reduced in Cav1^−/− eyes. Outflow facility was measured in perfused, enucleated eyes subjected to sequential pressure steps. Pressure-flow relationships (left panel) for Cav1^−/− and control eyes. The slope of the regression line (conventional outflow facility) is fit through the mean ± SEM values for n = 8 control and n = 9 Cav1^−/− eyes (from n = 5 control and n = 6 Cav1^−/− mice. (d) Comparison of conventional outflow facility between Cav1^−/− and control eyes (*p ≤ 0.05, unpaired two-tailed t-test).