Figure 4. Addition of adjuvant aflibercept or perioperative BowAng1 improves adjuvant paclitaxel chemotherapy for resected breast cancer.

18 days after orthotopic implantation of 2 × 10⁶ LM2-4 cells, mice bearing roughly 400-mm³ primary breast tumors were randomized into nine treatment groups. Primary tumor resections by complete mastectomies were performed at 22 days post-implantation (DPI). BowAng1 was given as a 10-day perioperative therapy beginning one day before surgery (21 DPI). Aflibercept (anti-VEGF-A/VEGF-B/PlGF), nesvacumab (anti-Ang2), the anti-Tie2 antibody, paclitaxel chemotherapy, and combinations thereof, were given as 4-week-long adjuvant therapies starting two days after surgery (24 DPI). Dosing schedule is depicted above survival curves. N = 7–9 mice per treatment group. Kaplan-Meier analyses of overall survival: (A) comparison of monotherapy groups; (B) comparison of combination therapies; and (C) showing only those single-agent and combination therapies that led to a statistically significant overall survival benefit compared to vehicle control and chemotherapy alone respectively (P < 0.05, log-rank test).