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. 2016 Nov 14;6:37160. doi: 10.1038/srep37160

Figure 1. Treatment with PF00915275 and C66 inhibited insulin resistance in high-fat fed mice.

Figure 1

PF00915275 (5 mg/kg) and C66 (5 mg/kg) were administered orally once every other day for 8 weeks to high fat diet-fed mice. (a) Body weight of HFD mice with or without PF00915275 or C66 treatment for 8 weeks showing that PF00915275 treatment reduced weight gain in mice fed HFD. (b) OGTT were performed on mice following the completion of all treatments and just before sacrifice. (c) Blood glucose AUC was calculated based on OGTT data presented in B. (d) protein levels of 11β-HSD1 and GR in subcutaneous adipose tissues from mice fed with normal chow or HFD was determined by western blot analysis. The gels were run under the same experimental conditions. Shown are cropped gels/blots (The gels/blots with indicated cropping lines are shown in Supplementary Fig. S1). Representative two mouse tissues from n = 8 were shown. (e–i) mRNA quantification of IRS-1 (e) GLUT (f) adiponectin (g) and FABP (h) by qPCR. (i) Akt phosphorylation levels measured in the adipose tissues. The gels were run under the same experimental conditions. Shown are cropped gels/blots (The gels/blots with indicated cropping lines are shown in Supplementary Fig. S1). Data was presented as mean ± S.E.M. *P < 0.05; **P < 0.01, ***P < 0.001 compared with the vehicle-treated group. n = 8 per group.