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. 2016 Nov 14;6:37160. doi: 10.1038/srep37160

Figure 2. Inhibition of 11β-HSD1 reduced JNK activation in 3T3-L1 cells.

Figure 2

(a,b) 3T3-L1 cells were pretreated with PF00915275 (10 μM) or vehicle (DMSO) for 2 h and then incubated with prednisone (5 μM) or DMSO for 1 h. Cell lysates were collected and 11β-HSD1 (a) and GR (b) were detected by western blotting. (c) Western blot analysis of JNK phosphorylation in adipocytes. Cells were treated as in A and B. (d) Cells with 11β-HSD1 overexpression were incubated with C66 (10 μM) or DMSO for 2 h. The expression of p-JNK/JNK was determined by western blot analysis. (e–g) Cells with 11β-HSD1 overexpression were incubated with TNF-α (1 μg/mL) or vehicle for 1 h. Cell lysates were collected and p-JNK/JNK (e), 11β-HSD1 (f) and GR (g) were detected by western blotting. (h,i) 11β-HSD1 overexpressig cells were incubated with C66 (10 μM) or DMSO for 2 h. The expression of 11β-HSD1 (h) and GR (i) was assayed. All the gels were run under the same experimental conditions. Shown are cropped gels/blots (The gels/blots with indicated cropping lines are shown in Supplementary Fig. S1). *P < 0.05, **P < 0.01, ***P < 0.001, all data from 3 independent experiments.