Figure 7. Increased expression level of CaM1 by full-length MG53 or PRY-SPRY.

(a) Lysate from the myotubes over-expressing full-length MG53 or PRY-SPRY was subjected to an immunoblot assay with one of the antibodies against nine proteins that are known to mediate skeletal EC coupling and/or the handling of Ca²⁺. α-Actin was used as a loading control. Three independent experiments per each protein were conducted. JP, junctophilin; CSQ, calsequestin. (b) Among them, the expression level of CaM1 (indicated by asterisks in a) is presented as bar graphs. Bar graphs are presented as the mean ± S.E. for three-independent experiments. *Significant difference versus Vector control (p < 0.05). Full-length blots are presented in Supplementary Figs 12 to 21.