Table 1. Intestinal key physiological functions and relevant differentially expressed proteins in the jejunal mucosa of pigs upon dietary protein limitation.
| UniProtKB accession no. | protein name | associated gene name | FC | function |
|---|---|---|---|---|
| Nutrient digestion, absorption, and metabolism | ||||
| up-regulated | ||||
| WAP3_PIG | protein WAP-3 | WAP-3 | 7.26 | peptidase inhibitor |
| C6L245_PIG | putative trypsinogen | TRY | 5.58 | protein digestion and absorption |
| F1RLV1_PIG | sodium/glucose cotransporter 1 | SGLT1 | 5.11 | digestive system process |
| F1SFI5_PIG | histidine-rich glycoprotein | HRG | 3.79 | cysteine-type endopeptidase inhibitor |
| DPEP1_PIG | dipeptidase 1 | DPEP1 | 2.96 | extracellular peptidase |
| down-regulated | ||||
| F1RG77_PIG | ring finger protein 40 | RNF40 | 0.27 | proteolysis involved in cellular protein catabolic process |
| F1SKG5_PIG | putative glycerol kinase 5 | GK5 | 0.32 | citrate cycle; glycerolipid metabolism |
| I3LRA1_PIG | lon peptidase 2 | LONP2 | 0.43 | serine-type endopeptidase activator (cytoplasm) |
| Immunity | ||||
| up-regulated | ||||
| F8J302_PIG | MHC class II antigen | SLA-DQB | 36.57 | immune response |
| GILT_PIG | gamma-interferon-inducible-lysosomal thiol reductase | IFI30 | 5.61 | antigen processing |
| L8AXK3_PIG | IgG heavy chain | IGHG | 4.57 | response to wounding; intestinal immune network for IgA production |
| K7GQR1_PIG | complement factor properdin | CFP | 4.06 | humoral immune and inflammatory response |
| Q9N2H7_PIG | poly-Ig receptor | PIGR | 3.92 | polymeric immunoglobulin receptor |
| down-regulated | ||||
| F1S514_PIG | COMM domain containing 7 | COMMD7 | 0.30 | negative regulation of NF-kappa B transcription factor activity |
| Q0MRZ8_PIG | MHC class I antigen | SLA | 0.41 | antigen processing; allograft rejection |
| C1QA_PIG | complement C1q subcomponent subunit A | C1QA | 0.46 | complement activation; innate immune response |
| H4_PIG | histone H4 | HIST4H4 | 0.47 | autoantigen component |
| F1RZ52_PIG | tripartite motif-containing protein 26 | TRIM26 | 0.47 | innate immune response; negative regulation of viral entry into host cell |
| Cell renewal | ||||
| up-regulated | ||||
| GILT_PIG | gamma-interferon-inducible-lysosomal thiol reductase | IFI30 | 5.61 | negative regulation of cell proliferation |
| F1SFI5_PIG | histidine-rich glycoprotein | HRG | 3.79 | negative regulation of cell growth and proliferation |
| Q9GJV4_PIG | four and a half LIM domains 1 protein isoform C | fhl1C | 3.66 | negative regulation of G1/S and G2/M transition of mitotic cell cycle |
| DPEP1_PIG | dipeptidase 1 | DPEP1 | 2.96 | negative regulation of apoptotic process and cell migration |
| F2Z5K4_PIG | ras homolog family member C | RHOC | 2.47 | negative regulation of programmed cell death |
| down-regulated | ||||
| F1RXX7_PIG | marker of proliferation Ki-67 | MKI67 | 0.34 | a marker of cellular proliferation |
| MEA1_PIG | male-enhanced antigen 1 | MEA1 | 0.45 | may play an important role in spermatogenesis and/or testis development |
| F1SGC0_PIG | FYVE, rhoGEF and PH domain containing 4 | FGD4 | 0.47 | involved in the actin cytoskeleton and cell shape |
| F1S436_PIG | fatty acid 2-hydroxylase | FA2H | 0.48 | regulation of cell proliferation |
| Regulation of DNA replication and gene expression | ||||
| up-regulated | ||||
| F1RM65_PIG | U2 small nuclear RNA auxiliary factor 1-like 4 | U2AF1L4 | 12.93 | pre-mRNA splicing factor |
| Q53DY7_PIG | histone H1.3-like protein | LOC595122 | 3.78 | nucleosome assembly |
| F2Z5E6_PIG | ribosomal protein S5 | RPS5 | 3.58 | RNA binding; regulation of translational fidelity |
| F1RIU9_PIG | coiled-coil-helix-coiled-coil-helix domain containing 2 | CHCHD2 | 3.17 | positive regulation of transcription from RNA polymerase II promoter |
| F1SA98_PIG | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta | YWHAQ | 3.06 | negative regulation of transcription |
| down-regulated | ||||
| F1SDG0_PIG | splicing factor 3b subunit 4 | SF3B4 | 0.46 | positive regulation of mRNA splicing |
| H4_PIG | histone H4 | HIST4H4 | 0.47 | nucleosome formation; chromatin organization |
| F1RZ52_PIG | tripartite motif-containing protein 26 | TRIM26 | 0.47 | positive regulation of sequence-specific DNA binding transcription factor activity |
| F1SPG1_PIG | histone H1x | H1FX | 0.49 | nucleosome assembly |
| ROCK2_PIG | rho-associated protein kinase 2 | ROCK2 | 0.50 | positive regulation of centrosome duplication |
FC, fold-change calculated by the abundance of a specific protein upon dietary protein limitation divided by it in control pigs.