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. 2016 Nov 14;6:37079. doi: 10.1038/srep37079

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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SbMATE2 exports dhurrin and other hydroxynitrile glucosides from X. Laevis oocytes, but not cyanidin 3-O-glucoside. The bars show the relative content of the indicated plant specialised metabolites in SbMATE2 expressing (right bars) and non-expressing control (left bars) oocytes 90 min after injection of the respective compounds into the oocytes (means, ±s.e., statistical significant differences to control oocytes are indicated *p < 0.05). Each of the compound solutions was injected into 25–30 oocytes to an estimated internal concentration of 100 μM. Following incubation, oocytes were analysed in triplicates consisting of 7–10 oocytes. The compounds shown are the cyanogenic glucosides dhurrin, prunasin, amygdalin, epiheterodendrin, the non-cyanogenic β-hydroxynitrile glucoside epidermin, indol-3-yl-methyl glucosinolate (I3M), and the anthocyanin cyanidin 3-O-glucoside (C3G).