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(a) EMSAs of SAV742 binding to intergenic regions of aveA1-aveD and aveA4-orf1, and the aveF promoter region. EMSA conditions as described for Fig. 2a. (b) qRT-PCR analysis of aveA1, aveD, aveA4, orf1, aveF and aveR in WT and D742 grown in FM-I. ***P < 0.001; NS, not significant (Student’s t-test).
