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. 2016 Nov 14;6:37027. doi: 10.1038/srep37027

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Control tumor tissue spotted with PTX standards at increasing concentration (R1 = 0 pmol, R2 = 0.5 pmol, R3 = 1 pmol, R4 = 2.5 pmol, R5 = 5 pmol, R6 = 10 pmol). The image shows the intensity of the PTX fragment ion at m/z 284.2 normalized to the D5-PTX fragment ion at m/z 289.2. (b) Calibration curve plotted using the mean signal intensity PTX/D5-PTX ratios of the region of interest (ROI) R2-R6. (c) 3D images of PTX/D5-PTX distribution inside treated mesothelioma with 2X magnification of the area with the higher signal.