Skip to main content
. 2016 Nov 14;6:36921. doi: 10.1038/srep36921

Figure 4. Expression of Cas9 and JCPyV specific gRNA mediated targeted cleavage in JCPyV genome.

Figure 4

(a) JCPyV genome from LentiCRISPR/gRNA stably expressing cells was extracted and the regions around the indicated target were amplified by PCR reactions. PCR products were clones and 50–70 colonies from each condition were sequenced. The chart showing the number of mutant colonies identified from the sequencing analysis. (b) Examples of deletions or mutations identified from the sequencing analysis for JCPyV genome from LentiCRISPR/gRNA-NCCR-a and LentiCRISPR/gRNA-VP1-b cells.