Table 1. The sequences and location of gRNA targeting JCPyV genome.

Name	JCPyV genomic target	Sense or anti-sense	Target location
NCCR-a	CAAGCATGAGCTCATACCTA	Sense	NCCR TandemRepeat (50, 148)
NCCR-b	TTTACTGGCTGTTAGCTGGT	Anti-sense	NCCR TandemRepeat (78, 177)
VP1-a	AAACCCCTAAGATGCTCATC	Anti-sense	VP1 ORF (1622)
VP1-b	GAAGTTTTGGAACTTGCACG	Anti-sense	VP1 ORF (1507)
VP2	GGTGCCGCACTTGCACTTTT	Sense	VP2 ORF (529)

Numbering is according to the Mad-1 strain of JCPyV (GeneBank accession no. NC_001699).