Table 1.

Primers and Probe for NIST-Developed Assays

Assay / Target / Locus	Primers and Probe	Anneal Temp
NEIF Gene EIF5B Chr 2 p11.1-q11.1	F gccaaacttcagccttctcttc R ctctggcaacatttcacactaca Pa tcatgcagttgtcagaagctg	62 °C
2PR4 Gene RPS27A Chr 2 p16	F cgggtttgggttcaggtctt R tgctacaatgaaaacattcagaagtct Pc tttgtctaccacttgcaaagctggccttt	60 °C
NR4Q Gene DCK Chr 4 q13.3-q21.1	F tggtgggaatgttcttcagatga R tcgactgagacaggcatatgtt Pa tgtatgagaaacctgaacgatggt	60 °C
Quantifiler (Qf) Gene hTERT Chr 5 p15.33	F proprietary R proprietary Pb proprietary	60 °C
D5 STR D5S2500 Chr 5 q11.2	F ttcatacaggcaagcaatgcat R cttaaagggtaaatgtttgcagtaatagat Pb ataatatcagggtaaacaggg	58 °C
ND6 STR D6S474 Chr 6 q21-22	F gcatggctgagtctaaagttcaaag R gcagcctcagggttctcaa Pa cccagaaccaaggaagatggt	60 °C
HBB1 Gene HBB Chr 11 p15.5	F gctgagggtttgaagtccaactc R ggtctaagtgatgacagccgtacct Pa agccagtgccagaagagccaagga	62 °C
ND14 STR D14S1434 Chr 14 q32.13C	F tccaccactgggttctatagttc R ggctgggaagtcccacaatc Pa tcagactgaatcacaccatcag	58 °C
22C3 Gene PMM1 Chr 22 q13.2	F cccctaagaggtctgttgtgttg R aggtctggtggcttctccaat Pc caaatcacctgaggtcaaggccagaaca	60 °C

bp: basepair, F: Forward primer, R: Reverse primer, P^a: Blackhole Plus quencher probe, P^b: Taqman MGB probe, P^c: Blackhole quencher probe, Anneal Temp: annealing temperature