. Author manuscript; available in PMC: 2017 Feb 16.

Published in final edited form as: Anal Chem. 2016 Jan 25;88(4):2132–2139. doi: 10.1021/acs.analchem.5b03692

Table 2.

Properties of Restriction Enzymes

Property	AluI	HaeIII	EcoRI	PstI
Recognition 5′- sequence 3′-	AG∣CT TC∣GA	GG∣CC CC∣GG	G∣AATTC CTTAA∣G	CTGCA∣G G∣ACGTC
End type	Blunt	Blunt	Sticky	Sticky
Cuts ssDNA?	No	Some	Yes	No
≈ No. Cut sites Mean fragment size	11.5 10⁶ 280 bp	5.1 10⁶ 630 bp	1.0 10⁶ 3100 bp	0.5 10⁶ 7000 bp
Units / mL	10,000	10,000	20,000	20,000