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Sample Preparation
| μL Component per 50 μL Solution | ||||
|---|---|---|---|---|
| Component | AluI | HaeIII | EcoRI | PstI |
| [Enzyme], 40 U | 4.0 | 4.0 | 2.0 | 2.0 |
| NEBuffer3 | 5.0 | |||
| NEBuffer4 | 5.0 | 5.0 | 5.0 | |
| BSA, 100 μg/mL | 0.5 | |||
| DNA, 2 μg | 2/[DNA] | 2/[DNA] | 2/[DNA] | 2/[DNA] |
| PCR-grade Water | 50-Σrest | 50-Σrest | 50-Σrest | 50-Σrest |
