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. Author manuscript; available in PMC: 2016 Nov 14.
Published in final edited form as: J Proteome Res. 2013 Feb 1;12(3):1289–1299. doi: 10.1021/pr3009435

Figure 2.

Figure 2

SPA tagged S12 (SPA-S12) pull-downs in whole cell lysates enriched for proteins representing a heterogeneous population of isolated 30S subunits, 70S ribosomes and/or translationally active polysomes of ribosomal small subunit associated complexes. Pull-downs performed in polysome depleted extract resulted in the enrichment of ribosomal small subunit associated complexes (A) Polypeptides from eluates representing SPA-S12 purified from whole cell lysates (lane 2) and negative control (lane 3) (B) Polypeptides from eluates representing SPA-S12 purified from polysome depleted extract (lane 2) and negative control (lane 3). Pull-downs were separated by SDS-PAGE (10% polyacrylamide gels) prior to in-gel tryptic digestion and ID LC tandem mass spectrometry. Molecular weight standards are represented in lane 1. The image representing (B) have been displayed in a previous report (18).