Table 2.

A. Ribosomal maintenance/translational/folding proteins from SPA-S12 pull-downs performed in whole cell lysates
Acession		Unique	Matched	% Seq.	Comment
	Interacting Protein	Peptides	Spectra	Cov.
P06992	RsmA	10	116	58.6	Ribo. RNA 30S methyltransferase A
P36929	RsmB	18	141	59.7	Ribo. RNA 30S methyltransferase B
P39406	RsmC	21	171	77.5	Ribo. RNA 30S methyltransferase C
P0ADX9	RsmD	4	19	27.7	Ribo. RNA 30S methyltransferase D
P0AGL7	RsmE	9	65	66.2	Ribo. RNA 30S methyltransferase E
P60390	RsmH	14	102	52.4	Ribo. RNA 30S methyltransferase H
P75876	Rlml	15	85	47	Ribo. RNA 50S methyltransferase I
P0AA39	RluC*	6	20	21.9	Ribo. 50S pseudouridine synthase C
P55135	RumA	5	12	15.5	23S rRNA (uracil-5-)-methyltransferase
P0AGJ5	yfiF*	14	174	46.4	Unchar. tRNA/rRNA methyltransferas
P76550	yffS	16	143	56.5	Uncharacterized protein
P0A8A8	RimP*	5	58	28.6	Ribosome maturation factor
P0A7X6	RimM*	9	37	58.2	Ribosome maturation factor
P43329	HrpA	43	312	41.4	ATP-dependent RNA helicase
P69222	IF-1	4	27	94.4	Translation initiation factor
P0A705	IF-2*	44	475	59.8	Translation initiation factor
P0A707	IF-3*	14	568	77.2	Translation initiation factor
P0A6M8	Elongation factor G	22	111	49.4	Elongation factor G
P21499	RNase R*	52	625	71.2	Ribonuclease R
P21338	RNase I*	11	86	66	Ribonuclease I
P30850	Exoribonuclease 2	11	29	21.1	Exoribonuclease 2
P0A9J0	RNase G	13	43	37	Ribonuclease G
P0A6X3	Protein hfq*	6	35	64.7	Protein hfq
P05055	PNP transferase *	15	58	29.8	Polyribonucleotide nucleotidyltransferase
P10408	SecA	22	64	35.8	Protein translocase subunit secA
P0A6Z3	HtpG	4	11	8.9	Chaperone protein htpG
P08622	DnaJ	6	22	23.7	Chaperone protein dnaJ
P0A850	Trigger factor*	16	99	47.9	Trigger factor
P63284	ClpB*	21	78	38.5	Chaperone protein clpB

B. Transcription proteins from SPA-S12 pull-downs performed in whole cell lysates
Acession	Protein	Unique	Matched	% Seq	Comments
	Name	Peptides	Spectra	Cov.
P0A7Z4	RpoA*	13	37	52.3	DNA-directed RNA polymerase subunit alpha
P0A8V2	RpoB*	37	142	36.9	DNA-directed RNA polymerase subunit beta
P0A8T7	RpoC*	47	290	41.5	DNA-directed RNA polymerase subunit beta'
P0A8W0	NanR	4	11	23.9	Transcriptional regulator
P0ACN4	AllR	8	61	35	HTH-type transcriptional repressor
P03030	LysR	5	11	27.6	Transcriptional activator protein
P37671	YiaJ	9	24	50.4	HTH-type transcriptional regulator
P0ACP5	GntR	10	35	43.8	HTH-type transcriptional regulator
P0AG30	Rho	6	20	17.9	Transcription termination factor
P39356	yjhU	3	6	12.2	Uncharacterized transcriptional regulator
P30864	YafC	8	35	28.3	Uncharacterized HTH-type transcriptional regulator
P77300	YagI	6	24	30.9	Uncharacterized HTH-tvpe transcriptional regulator

^*Proteins that were identified to be associated with the sucrose gradient purified ribosomes (36)–(35).

^*Proteins that were identified to be associated with the sucrose gradient purified ribosomes (35, 36).