Table 3.
A. Ribosomal proteins from SPA-S12 pull-downs performed in polysome-free (PDE) mid-log and stationary phase lysates | |||||||
---|---|---|---|---|---|---|---|
Midlog | Stationary | ||||||
Acession | Protein | Unique | Matched | % Seq. | Unique | Matched | % Seq. |
Name | Peptides | Spectra | Cov. | Peptides | Spectra | Cov. | |
P0AG67 | S1 | 26 | 176 | 50.8 | 42 | 417 | 93.7 |
P0A7V8 | S4 | 16 | 142 | 58.7 | 15 | 190 | 68.9 |
P0A7V3 | S3 | ** | 11 | 143 | 56.2 | ||
P0A7W1 | S5 | 12 | 114 | 82.6 | 12 | 323 | 41.7 |
P02358 | S6 | 8 | 80 | 62.9 | 6 | 24 | 61.5 |
P02359 | S7 | 15 | 147 | 69.8 | 14 | 240 | 60.8 |
P0A7W7 | S8 | 8 | 82 | 67.7 | 10 | 170 | 79 |
P0A7X3 | S9 | 6 | 58 | 42.3 | 8 | 133 | 48.4 |
P0A7R5 | S10 | 5 | 31 | 43.7 | 9 | 114 | 53.4 |
P0A7R9 | S11 | 7 | 29 | 79.8 | 5 | 56 | 50 |
P0A7S3 | S12 | 6 | 71 | 24.2 | 8 | 96 | 55.6 |
P0A7S9 | S13 | 9 | 60 | 61 | 9 | 92 | 67.8 |
P0ADZ4 | S15 | 8 | 90 | 68.5 | 8 | 36 | 60 |
P0A7T7 | S18 | 5 | 63 | 50.6 | 5 | 99 | 50.6 |
P0A7U7 | S20 | 4 | 22 | 55.2 | ** |
B. Candidate S12 binders from polysome-free (PDE) mid-log and stationary | |||||||
---|---|---|---|---|---|---|---|
Midlog | Sationary | ||||||
Acession | Protein Name | Unique | Matched | % Seq. | Unique | Matched | % Seq. |
Peptides | Spectra | Cov. | Peptides | Spectra | Cov. | ||
P0AEI4 | MiaB like Methyltransferase RimO | 9 | 34 | 24.3 | ** | ||
P75838 | uncharacterized ycaO | 21 | 237 | 44.3 | 13 | 103 | 24.5 |
P76116 | uncharacterized yncE | 10 | 44 | 38.8 | 4 | 11 | 18.4 |
P33355 | uncharacterized yehS | 7 | 22 | 50 | ** | ||
P0AF93 | uncharacterized yjgf | 7 | 52 | 77.4 | 8 | 32 | 69.5 |
P0CE47 | Elongation factor Tu | 34 | 1370 | 84.5 | ** | ||
P0A6P1 | Elongation factor Ts | 16 | 52 | 55.8 | ** | ||
P0A6M8 | Elongation factor G | 43 | 482 | 73 | 25 | 131 | 53.8 |
P0A6N4 | Elongation factor P | 5 | 17 | 26 | ** | ||
P21499 | RNaseR | 22 | 67 | 41.2 | 31 | 145 | 50.5 |
P0A6X3 | Hfq-1 | 7 | 25 | 54 | 5 | 20 | 44 |
P0A805 | Ribosome Recycling Factor | 6 | 21 | 47 | 5 | 12 | 35.7 |
P0A8A8 | Ribosome maturation factor rimP | 6 | 40 | 33 | 6 | 80 | 35.3 |
Protein was either not detected at all or was below the baseline level.