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. Author manuscript; available in PMC: 2016 Nov 14.
Published in final edited form as: J Proteome Res. 2013 Feb 1;12(3):1289–1299. doi: 10.1021/pr3009435

Table 3.

A. Ribosomal proteins from SPA-S12 pull-downs performed in polysome-free (PDE) mid-log and stationary phase lysates
Midlog Stationary
Acession Protein Unique Matched % Seq. Unique Matched % Seq.
Name Peptides Spectra Cov. Peptides Spectra Cov.
P0AG67 S1 26 176 50.8 42 417 93.7
P0A7V8 S4 16 142 58.7 15 190 68.9
P0A7V3 S3 ** 11 143 56.2
P0A7W1 S5 12 114 82.6 12 323 41.7
P02358 S6 8 80 62.9 6 24 61.5
P02359 S7 15 147 69.8 14 240 60.8
P0A7W7 S8 8 82 67.7 10 170 79
P0A7X3 S9 6 58 42.3 8 133 48.4
P0A7R5 S10 5 31 43.7 9 114 53.4
P0A7R9 S11 7 29 79.8 5 56 50
P0A7S3 S12 6 71 24.2 8 96 55.6
P0A7S9 S13 9 60 61 9 92 67.8
P0ADZ4 S15 8 90 68.5 8 36 60
P0A7T7 S18 5 63 50.6 5 99 50.6
P0A7U7 S20 4 22 55.2 **
B. Candidate S12 binders from polysome-free (PDE) mid-log and stationary
Midlog Sationary
Acession Protein Name Unique Matched % Seq. Unique Matched % Seq.
Peptides Spectra Cov. Peptides Spectra Cov.
P0AEI4 MiaB like Methyltransferase RimO 9 34 24.3 **
P75838 uncharacterized ycaO 21 237 44.3 13 103 24.5
P76116 uncharacterized yncE 10 44 38.8 4 11 18.4
P33355 uncharacterized yehS 7 22 50 **
P0AF93 uncharacterized yjgf 7 52 77.4 8 32 69.5
P0CE47 Elongation factor Tu 34 1370 84.5 **
P0A6P1 Elongation factor Ts 16 52 55.8 **
P0A6M8 Elongation factor G 43 482 73 25 131 53.8
P0A6N4 Elongation factor P 5 17 26 **
P21499 RNaseR 22 67 41.2 31 145 50.5
P0A6X3 Hfq-1 7 25 54 5 20 44
P0A805 Ribosome Recycling Factor 6 21 47 5 12 35.7
P0A8A8 Ribosome maturation factor rimP 6 40 33 6 80 35.3
**

Protein was either not detected at all or was below the baseline level.