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. 2016 Oct 4;186(3):373–386. doi: 10.1111/cei.12859

Figure 2.

Figure 2

Increasing coefficient of variation (CV) of glycosylphosphatidylinositol‐anchored 80 kD protein (GPI‐80) expression through treatment with a combination of granulocyte‐colony stimulating factor (G‐CSF) with granulocyte–macrophage (GM)‐CSF. HL60 cells were differentiated by 1·25% DMSO in the presence of 1 nM G‐CSF or GM‐CSF, or a combination of G‐CSF and GM‐CSF for 4 days. The differentiated cells were measured by flow cytometry, and the mean fluorescence intensity (MFI) and coefficient of variation (CV) of GPI‐80 in CD16+ cells were analysed. Percentage of increase in GPI‐80 MFI was calculated as follows: % increase of GPI‐80 MFI = [(GPI‐80 MFI treated with G‐CSF, GM‐CSF or combination) – (GPI‐80 MFI treated with vehicle)/(GPI‐80 MFI treated with vehicle] × 100. CV was calculated automatically as robust CV by FlowJo software using the following formula: CV = 100 × ½ (intensity at 84·13 percentile – intensity at 15·87 percentile)/median. *P < 0·05; **P < 0·01; ***P < 0·001 compared to vehicle (n = 4).