Figure 6.

Inverse correlation between glycosylphosphatidylinositol‐anchored 80 kD protein (GPI‐80)⁺ coefficient of variation (CV) and T cell proliferation. (a) Representative histograms of carboxyfluorescein diacetate succinimidyl ester (CFSE)‐labelled CD3⁺ T cell proliferation assay, analysed by flow cytometry, are shown. The upper panel is the result from patients and the lower panel is from healthy volunteers. (b) GPI‐80 CV in CD16^hi population in peripheral blood was measured (vertical axis) and CFSE‐labelled T cells were incubated with autologous polymorphonuclear leucocytes [polymorphonuclear cells (PMN), mainly CD16^hi population]; the ratio of T cells to PMN was adjusted to 1 : 1. After incubation, the percentage of proliferating T cells was measured (horizontal axis). The results from the patients are indicated as closed circles and the results from healthy volunteers are shown as open circles.