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. 2016 Oct 4;186(3):292–303. doi: 10.1111/cei.12857

Figure 6.

Figure 6

Lack of exacerbation of collagen type II‐induced arthritis (CIA) in B10.RIII. low‐density lipoprotein receptor (LDLR–/–) mice; 8–12‐week‐old B10.RIII.wild‐type (WT), B10.RIII.LDLR –/– and B10.RIII.apolipoprotein E (ApoE –/–) mice fed with a normal chow diet (NCD) or an atherogenic hypercholesterolaemic diet (HCD) were immunized with collagen type II‐complete Freund's adjuvant (col II‐CFA). (a) Clinical severity of CIA in individual mice 8 weeks after immunization with col II. Bars represent the mean values. (b) Representative radiological images of the front paws from mice before immunization with col II and 8 weeks afterwards. (c) Severity score of individual radiological signs, expressed as the mean ± standard deviation (n = 6–8 mice/group) 8 weeks after immunization. Bars represent the mean values. Results from (a) to (c) are representative of three independent experiments. (d) Expression of interleukin (IL)‐1β, tumour necrosis factor (TNF)‐α and IL‐6 mRNAs by reverse transcription–quantitative polymerase chain reaction (RT–qPCR) in the paws of the mice fed with NCD (open bars) or HCD (closed bars) 8 weeks after (Imm) immunization with col II. For each cytokine analysis, a mixed group of non‐immunized (NI) mice is included for comparison. Representative results from one of three independent experiments (five to nine animals/group) are expressed as mean ± standard deviation fold change of each cytokine relative to glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) expression measured in parallel in each sample. Statistical differences are indicated as follows: n.s. = non‐significant; *P < 0·05; **P < 0·01; ***P < 0·005.