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. Author manuscript; available in PMC: 2016 Nov 14.
Published in final edited form as: Cell Rep. 2016 Oct 25;17(5):1302–1317. doi: 10.1016/j.celrep.2016.10.006

Figure 2. SIRT2 regulates Slug protein abundance and activity.

Figure 2

(A) (Left) Immunoblots showing levels of Slug, V5 tagged SIRT2 (SIRT2-V5) and Lamin A/C in MCF10A cells ectopically expressing SIRT2-V5 or control lacZ. (Right) Quantification of relative Slug levels from five independent experiments, normalized to Lamin A/C. Data are shown as mean ± SEM. * p < 0.05.

(B) (Left) Immunoblots showing levels of Slug, SIRT2, acetylated tubulin (a bona fide SIRT2 substrate) and Lamin A/C in MCF10A cells expressing two different hairpins targeting SIRT2. (Right) Quantification of relative Slug levels from three independent experiments, normalized to Lamin A/C. Data are shown as mean ± SEM. ** p < 0.01.

(C) (Top) Immunoblots showing levels of Slug, SIRT2-V5, Cyclin D1 and Lamin A/C in CHX-treated MCF10A cells overexpressing SIRT2-V5 or control LacZ. (Bottom) Quantification of relative Slug levels from three independent experiments, normalized to Lamin A/C. Data are shown as mean ± SEM. * p < 0.05.

(D) (Top) Immunoblot showing levels of Slug, SIRT2-V5 and Lamin A/C in CHX-treated MCF10A cells expressing either shSIRT2 or a non-silencing shRNA control (shCtrl). (Bottom) Quantification of relative Slug levels from three independent experiments, normalized to Lamin A/C. Data are shown as mean ± SEM. * p < 0.05.

(E) Expression of Slug target genes EPCAM and CDH1 in MCF10A cells overexpressing SIRT2 or LacZ (n = 3) as determined by qRT-PCR. Data are shown as mean ± SEM. * p < 0.05.

(F) Expression of Slug target genes EPCAM and CDH1 in MCF10A cells expressing shSIRT2 or shCtrl (n = 3) as determined by qRT-PCR. Data are shown as mean ± SEM. ** p < 0.01.