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. 2016 Aug 1;229(6):871–891. doi: 10.1111/joa.12526

Figure 4.

Figure 4

C9orf72 is strongly expressed in the molecular layer of the developing and adult cerebellum. Expression of C9orf72 in the developing cerebellum from E18.5 to P35 (A). C9orf72 staining is not seen in the neurofilament‐positive internal structures at any stage, but is found strongly at the inner edge of the developing external granule layer (EGL) at E18.5, which then goes onto to expand as the molecular layer (ML) expands, between the EGL and calbindin (CalB)‐positive Purkinje cell bodies (pc) adjacent to the forming inner granule layer (IGL). Scale bars: 500 μm. Throughout cerebellar development (B) C9orf72 expression in the molecular layer is not associated with Bergmann glia (GFAP) or proliferating granule cells (PCNA) but does associate with Purkinje dendrites (CalB) and synapses (SV2). C9orf72 appear strongest at the most superficial part of the molecular layer. Scale bars: 50 μm.