Table 2.

Mean ± SD methylation rates in the reproductive females and in the soldiers of Experiment 1. To facilitate comparison with methylation rates reported for other species, global methylation frequencies were calculated in two ways: as the proportion of methylated fragments out of all loci (second data column in the Table) and as the proportion of methylated fragments out of the methylation‐sensitive loci (column 3). Methylation levels of hemimethylated and internal cytosines are reported separately (columns 4‐5), as recommended by Alonso et al. (2016)

Caste	Global (1) (# methylated loci/(# MSL+# NML))	Global (2) (# methylated loci/# MSL)	Hemimethylated cytosines/# MSL	Internal cytosines/# MSL
Reproductive	0.153 ± 0.056	0.199 ± 0.074	0.061 ± 0.025	0.138 ± 0.066
Soldier	0.191 ± 0.109	0.249 ± 0.142	0.171 ± 0.134	0.078 ± 0.034