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. Author manuscript; available in PMC: 2016 Nov 14.
Published in final edited form as: Methods Mol Biol. 2016;1460:129–140. doi: 10.1007/978-1-4939-3810-0_10

Table 1.

Reagents for fluroscent labeling of skeletal muscle

Antigen Company Cat # Concentration Comment
CD31-Alexa 488 Biolegend 102514 5–10 µg for
intravital		 Marks blood vessels. Does not
work on fixed tissue
DyLight 488 or 649
labeled tomato lectin		 Vector
staining		 DL-1174,
DL-1177		 70 µg for
intravital
1:50 for whole
mount		 Marks blood vessels.
Fluorescently labelled
fixable dextran or Isolectin
B4 may also be used
Laminin Sigma-
Aldrich		 L-9393,
L-0663		 1:100 for
whole mount		 Marks muscle membrane and
blood vessels
Dystrophin Abcam Ab15277 1:100 for
whole mount		 Marks skeletal muscle
membranes
Alexa Fluor® conjugated
secondary antibodies		 Thermo
Fisher
Scientific		 1:200 Classical fluorescence dyes are
not recommended
Donkey/goat/sheep
serum		 Sigma-
Aldrich		 5 % Block based on the species
the secondary antibody is
from (see Note 1)