Figure 6.

Protein fraction in DRibbles is important for IL-1β induction. (a) Apyrase, RNase and DNase; (b) Proteinase K, trypsin, papain and 72 °C heat; (c) 37, 55, 72 °C water bath; (d) HSP90 inhibitor geldanamycin (GA) and Novobiocin treated for 30 min before DRibbles were incubated with primed THP-1 overnight. (e) Primed/unprimed THP-1 were stimulated with GA-treated/untreated 100 μg/ml DRibbles overnight. The supernatant IL-1β was tested by ELISA. Results are shown as the means+S.D. and representative of two or more independent experiments