Figure 6.

Infection with VACV or MCMV leads to enhanced cell death in Bcl-X_L^−/− macrophages but Mcl-1^−/− macrophages are similarly or less sensitive to apoptosis compared with wt. On day 7 of differentiation, macrophages wt or Mcl-1^−/− (a and c) or wt or Bcl-X_L^−/− (b and d) were infected with VACV for 16 h (MOI=10) (a and b) or MCMV for 24 h (MOI=5 or 10) (c and d). Cell death was measured as the percentage of cells stained positive for the Far Red dye of the Dead Cell Stain Kit. Data show means/S.E.M. of three (a), four (b and c) or five (d) experiments (***P=0.0006 (a); ***P=0.00007 (b); ns, P=0.07 (c, MOI=5); ns, P=0.15 (c, MOI=10); ***P=0.0005 (d, MOI=5); **P=0.004 (d, MOI=10); ns, not significant; two-tailed unpaired t-test)