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. 2016 Aug 25;7(8):e2347. doi: 10.1038/cddis.2016.243

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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LAMP-1 and 2 are substrates of FUT1. (a) Expression levels of LeY antigens on immunoprecipitated LAMP-1 and 2 from control, FUT1 and FUT2 knockdown cells. Both LAMP-1 and 2 were immunoprecipitated from FUT1 and FUT2 silenced T47D cells and then immunobloted with antibodies against LeY. The same blots were stripped and reprobed for LAMP-1 or LAMP-2. The ratios of LeY to immunoprecipitated LAMP-1 or LAMP-2 were quantified and fold changes relative to control were shown as indicated. (b) Immunoblot analysis of LeY levels in MCF-7, T47D and highly metastatic MDA-MB-231 cells (left panel). Actin was used as loading control. Expression levels of LeY antigens on immunoprecipitated LAMP-1 and 2 from MCF-7, T47D and MDA-MB-231 cells (right panel). LAMP-1 and 2 were immunoprecipitated from the indicated cells, and immunobloted with anti-LeY, anti-LAMP-1 or anti-LAMP-2 antibodies used in (a)