Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 25;7(8):e2346. doi: 10.1038/cddis.2016.245

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

RIPK3 has a positive effect on the activation of Caspases in the absence of NEMO. (a) siCTR and siRIPK3 Nemo⁽⁻⁾ MEF cells were treated with TNF (10 ng/ml) and Caspase-3/7 activation was measured in function of time (h). Error bars represent S.D. of quadruplicates of one representative experiment. Immunoblot analysis was performed to determine the RIPK3 protein level. (b) MEFs Nemo⁽⁻⁾ or Nemo⁽⁺⁾ were stimulated with TNF (10 ng/ml) for 3 h. Immunoblot analysis was performed to determine the indicated protein levels. (c and d) MEFs Nemo⁽⁻⁾ cells were pre-treated with Nec1 for 1 h and then stimulated with TNF (10 ng/ml) alone or in combination with zVAD, for the indicated time. Immunoblot analysis was performed to determine the indicated protein levels