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. 2016 Jun 30;7(6):e2282. doi: 10.1038/cddis.2016.170

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Copyright © 2016 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Conditional deletion of Tfrc in NCCs does not affect neural crest migration or cell proliferation. (a and b) Whole-mount AP-2α staining shows migratory neural crest cells in FNP (asterisk), BA1 (arrow) and BA2 (arrowhead). (c and d) Whole-mount X-gal staining shows neural crest cells in FNP (asterisk), BA1 (arrow) and BA2 (arrowhead). (e and f) Immunofluorescent labeling (e) and quantification (f) of BrdU-positive cells in mandibular regions from coronal head sections. At least twenty-five sections were randomly selected from four pairs of E13.5 Wnt1^cre;Tfrc^f/f mutants and controls. NS, no significant difference. Scale bar, 0.5 mm (a–d), 0.1 mm (e). BA1, first branchial arch; BA2, second branchial arch; FNP, frontonasal prominence