Fig. 2.

Median-joining phylogenetic NETWORKs illustrating Eimeria tenella SNP haplotype diversity assessed using (A) 52 SNP Sequenom MassARRAY or (B) 11 SNP PCR-RFLP genotyping protocols. Both illustrate a regional bias in haplotype occurrence with a small number of highly represented haplotypes detected in North Africa and northern India, and a much larger number of mostly unique haplotypes in southern India and Nigeria. Haplotypes from the UK and Ireland were only available using PCR-RFLP and appear distinct from the other regions. Note the incomplete NETWORK resolution achieved using the smaller number of PCR-RFLPs, indicated by multiple cycles or cubes. Node size indicates the frequency of haplotype occurrence. Panel A reproduced from (Blake et al., 2015).