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Chronic alcohol ingestion in vivo decreased Trx1 gene and protein expression in the whole lung. Control‐fed and alcohol‐fed mice were sacrificed after 8 weeks of feeding. The lungs were harvested and analyzed for (A) Trx1 mRNA expression by quantitative PCR and (B) Trx1 protein expression by Western immunoblot (representative gels shown above summary data). *p < 0.05 decreased compared to lungs from control‐fed mice. N = 5 to 6.
