Figure 2.

Alcohol exposure in vitro decreased Trx1 protein expression in lung fibroblasts. 3T3 lung fibroblasts were cultured ± alcohol (60 mM), and protein was isolated at 72 hours. (A) Total protein was analyzed for Trx1 expression by Western immunoblot (representative gels shown above summary data). (B) Total protein was separated into nuclear and cytosolic fractions and analyzed for Trx1 protein expression by Western immunoblot analyses (normalized to GAPDH in the cytosolic fraction and to histone in the nuclear fraction; representative gels shown above summary data). *p < 0.05 decreased compared to Trx1 expression in the cytosolic protein from unexposed cells (no alcohol). **p < 0.05 decreased compared to Trx1 expression in the nuclear protein from unexposed cells. N = 5 to 7.