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. 2016 Jul 20;40(9):1846–1856. doi: 10.1111/acer.13148

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Copyright © 2016 The Authors Alcoholism: Clinical and Experimental Research published by Wiley Periodicals, Inc. on behalf of Research Society on Alcoholism

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Nuclear overexpressing Trx1 (NLS‐Tg) mice were protected from alcohol‐mediated fibroproliferative disrepair following bleomycin‐induced lung injury. 3‐month‐old WT and NLS‐Tg littermates were treated ± alcohol (20% v/v in water for 8 weeks) then treated with 2 units/kg bleomycin intratracheally. Lungs were harvested at 14 days and analyzed for (A) histological changes by Masson's trichrome staining, (B) morphological analyses for collagen deposition, and (C) actual and (D) relative hydroxyproline content per mg of the right upper lobe of the lung. *p < 0.05 increased compared to untreated WT. N = 4 to 6.