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. 2016 Jul 20;40(9):1846–1856. doi: 10.1111/acer.13148

Figure 6.

Figure 6

Alcohol failed to suppress Nrf2‐ARE activity in PLF isolated from NLS‐Tg mice. PLF were isolated from WT and NLS‐Tg mice; cells between passages 3 to 5 were used. (A) Cells were transfected with ARE‐luciferase or Renilla luciferase for 24 hours and then cultured ± alcohol (60 mM) for 20 hours at which time relative Nrf2‐ARE activity (Nrf2 luciferase activity normalized to Renilla luciferase activity) was quantified. (B) Cells were cultured ± alcohol (60 mM) for 24 hours and then analyzed for GCLC and GSTT2 gene expression by quantitative PCR of mRNA. *p < 0.05 decreased compared to untreated WT. N = 7 to 10.