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Spectrophotometric measurement of TLR2, TLR4, and TLR5 activation in HEK 293 cell lines.
Notes: HEK were stimulated with ES0 (3–100 ng/mL) or positive controls: Pam2CSK4 = TLR2 ligand (A), LPS = TLR4 ligand (B), and flagellin = TLR5 ligand (C). ***P<0.001 versus control; data are representative of three independent experiments.
Abbreviations: ES0, extract from A. dolomiae; HEK, human embryonic kidney; LPS, lipopolysaccharide; OD, optical density; TLR, toll-like receptor.
