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. 2016 Nov 10;9:421–434. doi: 10.2147/CCID.S113180

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© 2016 Aries et al. This work is published and licensed by Dove Medical Press Limited

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Relative quantity of hBD-2, CAMP, and psoriasin mRNA in NHK cells.

Notes: NHKs were stimulated by IL-1β (20 ng/mL, positive control), ES0 (6.5 µg/mL), flagellin (100 ng/mL), or alone (negative control). Pretreatment was performed for 1 h with anti-TLR2 ( ), anti-TLR4 ( ), anti-TLR5 (■) (1 µg/mL), or without antibody (□) to test whether stimulation was blocked in ES0- and flagellin-treated samples; data are the mean of three independent experiments. ^*P<0.05; ^**P<0.01; ^***P<0.001 versus control or comparator.

Abbreviations: CAMP, cathelicidin antimicrobial peptide; ES0, extract from A. dolomiae; hBD2, beta-defensin 2; NHK, normal human keratinocyte; TLR, toll-like receptor.