Table 2.
Analysis of the reaction of di-Zn(II) and di-Fe(II) BcII with nitrocefin in a 1:1 ratio. Fitting constants are obtained from analysis of reaction time courses seen in Fig. 1 & Figs. S4–5. Substrate decay, product accumulation and intermediate accumulation and decay correspond to absorbance features at 390, 485 and 665 nm, respectively.
| Metal | pH | Substrate decay (s− 1) | Intermediate accumulation (s− 1) | Intermediate decay (s− 1) | Product accumulation (s− 1) |
|---|---|---|---|---|---|
| Zn(II) | 5.5 | 4.16 ± 0.01 | – | – | 4.13 ± 0.01 |
| Zn(II) | 6.5 | 3.24 ± 0.01 | – | – | 3.29 ± 0.01 |
| Zn(II) | 7.5 | 2.55 ± 0.01 | – | – | 2.75 ± 0.01 |
| Fe(II) | 5.5 | 3.88 ± 0.01 | Not fitted | Not fitted | 5.78 ± 0.01 |
| Fe(II) | 6.5 | 5.25 ± 0.01 | 18.31 ± 0.01 | 1.2 ± 0.1 | 4.27 ± 0.01 |
| Fe(II) | 7.5 | 7.25 ± 0.01 | 20.32 ± 0.01 | 1.4 ± 0.1 | 7.20 ± 0.01 |