Figure 1.
Ang-(1-7) administration in established cancer-induced bone pain attenuates spontaneous pain. Spontaneous pain behaviors (A) guarding and (C) flinching were recorded in a 2-minute period 14 days after inoculation and at various time points after drug administration. Time of peak effect at 15 minutes after administration (P < 0.01 for 0.36 and 1 μg/kg, compared with saline) lasting 2 hours. (B) Emax = 52.75%, A90 = 0.058 μg/kg for the guarding curve, (D) A90 = 1 μg/kg for the flinching curve. Tactile allodynia was measured 14 days after inoculation and at various time points after drug administration; a significant (P < 0.05, n = 8) reduction in evoked pain behaviors with an onset 30 minutes was measured after injection of 0.360 and 1 μg/kg and persisted for nearly 2 hours (E). Area under the curve was calculated (F). Values represent mean ± SEM, n = 8 per group.