Skip to main content
. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Mitochondrion. 2016 Sep 17;31:33–39. doi: 10.1016/j.mito.2016.09.001

Figure 4. Effect of loss of p53 on mitochondria activity in leukemic splenocytes of TCL1 mice.

Figure 4

A, Basal oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) was measured inleukemic splenocytes isolated from TCL1/p53+/+ mice (n=4) and TCL1/p53−/−mice (n=5). Three measurement cycles in duplicate was taken for each mouse sample. Representative graphs for OCR and ECAR are shown. Dotted lines indicate the time points of injection of 1 μM oligomycin (Oligo), 4 μM FCCP, or 2μM Rotenone (Rot). B, Comparison of the mean basal OCR between TCL1/p53+/+ mice (n=4) and TCL1/p53−/− mice (n=5). C, Comparison of the mean ECAR between TCL1/p53+/+ mice (n=4) and TCL1/p53−/− mice (n=5). D, Cellular ATP levels in the splenocytes from TCL1/p53+/+ and TCL1/p53−/− mice (n=4). E, Comparison between TCL1/p53+/+ mice (n=4) and TCL1/p53−/− mice (n=5) for their relative OCR normalized by mitochondrial mass measured by MitoTracker Green staining.