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. Author manuscript; available in PMC: 2017 Dec 15.
Published in final edited form as: Bioorg Med Chem. 2016 May 14;24(24):6276–6290. doi: 10.1016/j.bmc.2016.05.021

Figure 5.

Figure 5

JBIR-100 depolarizes the B. subtilis ATCC 47096 cellular membrane. Red/green ratios were calculated using mean fluorescence intensities of cells treated for 30 min at ~22 °C with 0.1 µM DiOC2(3) and vehicle (DMSO) (negative control), 5.0 µM CCCP (positive control), 0.1 µM JBIR-100, 0.2 µM JBIR-100, 0.5 µM JBIR-100, 1.0 µM JBIR-100. Data were normalized to the negative control sample and are depicted from three independent replicates. Error is given as standard deviation with n = 3. P-values are given relative to the negative control with * indicating < 0.05 and ** indicating < 0.005. Abbreviations: DiOC2(3), 3,3′-diethyloxacarbocyanine iodide; CCCP, carbonyl cyanide m-chlorophenyl hydrazone.