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. Author manuscript; available in PMC: 2017 Dec 4.
Published in final edited form as: J Ethnopharmacol. 2016 Aug 21;193:293–302. doi: 10.1016/j.jep.2016.08.032

Fig. 2.

Fig. 2

Impaired lymphatic thoracic duct formation induced by VEGFR-3 kinase inhibitor (MAZ51) was rescued by PNS in concentration dependent manner. The 48 hpf zeberafish (fli1:egfp; gata1:dsred) was treated with 30μM MAZ51 for 6 hours and then changed to be treated with different concentrations of PNS (10,50, 100 μM) for 48 hours. Embryos treated with 0.2% DMSO served as a vehicle control. (A) Representative confocal images show that PNS increased lymphatic thoracic duct formation of zeberafish, white arrow indicates lymphatic thoracic duct, and white star indicates lack of lymphatic vessel. Scale bars, 100 μm. (B) Quantitation of the length of lymphatic thoracic duct. Values are mean ± SD of 9–11 zeberafishes. *P<0.05 vs. control group; # P<0.05 versus MAZ51 treated group.