Fig. 4. Effects of kainate on expression of phosphacan (scale bar, 75 µm). Western blot was performed (n=3–6 for immunoblot analysis at each time point in each group). Stained GFAP and phosphacan representing astrogliosis were the largest in the scratch/kainate (S/K)-induced injury model among the control (CON), scratch (S), or S/K-induced injury models. Merged images (Merged) showed double-immunolabeling for GFAP and phosphacan. GFAP, glial fibrillary acidic protein; CNQX, 6-cyano-nitroquinoxaline-2,3-dione.