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. 2016 Nov 15;7:146. doi: 10.3389/fendo.2016.00146

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Copyright © 2016 Dauven, Ehrentraut, Langwisch, Zenclussen and Schumacher.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Treatment with rhCG did not alter cytokine secretion by activated BMDCs. BMDCs were stimulated with LPS/IFN-γ in the presence or absence of different concentrations of rhCG for 24 h. Unstimulated BMDCs served as controls. Secretion of IL-6 (A), IL-10 (B), IL-12p70 (C), MCP-1 (D), and TNF (E) was determined by CBArray analysis via flow cytometry. rhCG treatment did not influence the cytokine secretion profile of BMDCs. Each assay was repeated five times in duplicates. Data are presented as means plus SEM. Statistical analysis was carried out by one-way ANOVA followed by Bonferroni correction for multiple comparisons. **p < 0.01, ***p < 0.001.