Figure 7.
Adoptive transfer of hCG-treated BMDCs into abortion-prone females prior to mating augmented decidual IL-10 and TGF-β expression. BMDCs were stimulated with LPS/IFN-γ in the presence or absence of 500 mIU/ml rhCG for 24 h. Afterward, 1 × 106 BMDCs were adoptively transferred into virgin animals immediately before mating. On day 12 of pregnancy, females were sacrificed and decidual expression of TGF-β and IL-10 was assessed by RT-PCR. Transfer of hCG-treated BMDCs increased the expression of TGF-β (A) and IL-10 (B) when compared to females treated with mature BMDCs. Data are presented as medians. Individual values are displayed for each animal. Statistical analysis was carried out by using the non-parametric Mann–Whitney U test. *p < 0.05.