Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 15;10:111. doi: 10.3389/fnana.2016.00111

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Johnson Chacko, Pechriggl, Fritsch, Rask-Andersen, Blumer, Schrott-Fischer and Glueckert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

(A) PAX2 expression in a crista ampullaris at W9 with staining apparent in the nuclei and cytoplasm of the hair cells as well in the DC's (B) PAX2 exhibits intense cytoplasmic expression in the saccule and epithelial cells of the membranous labyrinth. (C) Intense and specific staining is apparent at the VG neurons at W11 with different intensities in cell nuclei. (D) PAX2 expression in an ampullary crest at W12, hair cells and the DC are positive for this marker. However, the TC is negative. There is a gradient of lower staining toward the periphery. The semi-circular canals side (SCC) (arrow head) shows less immunoreactivity compared the utricular side of the crest. (E) PAX8 immunostaining is visible in the otic epithelia among the future hair cells of the cristae ampullaris at W8. (E, inset) the staining for PAX8 is also visible in the developing cochlea where portions of the Lesser epithelial ridge (LER) and the future Organ of Corti are immunopositive for this marker. (F) PAX8 expression at W12 is confined to the hair cells of the ampullary crests and the utricle. More evident is the PAX8 immunoreactivity among the hair cells of the utricle (G) at higher magnification. (F, inset) image is the basal turn of the developing cochlea at W12 were clearly visible staining for PAX8 is evident in the Outer hair cells (OHC), Inner hair cells (IHC) as well as stria marginal cells and the Reissner's membrane (arrows). (H) PAX6 expression is visible in all epithelial as well as mesemchymal cells at W9. Only the differentiated hair cells are void of any immunoreactivity. (I) PAX6 immunoreactivity at W9 in the satellite glia and Schwann cells of the vestibular ganglion. (J) PAX6 immunoreactivity is no longer apparent at W12, satellite glial cells show a more adult-like morphology indicating advancement in the VG developmental process. Scale bar: 12 μm (A–D,G), 50 μm (F), 10 μm (E) 8 μm (H–J and insets of E,F).